Journal: Scientific Reports

Article Title: FTY720 in resistant human epidermal growth factor receptor 2-positive breast cancer

doi: 10.1038/s41598-021-04328-y

Figure Lengend Snippet: Xenograft mouse model of HCC1954 cells showing the efficacy of FTY720 with or without trastuzumab. ( a ) Mice bearing HCC1954 xenografts were randomized into four treatment groups (n = 9 for each group) when tumor size reached 150–200 mm 3 . Mice were treated with PBS, trastuzumab, FTY720, or FTY720 plus trastuzumab. Xenografts were harvested 24 h after the last treatment. Each plot indicates the mean increase in tumor volume from the first day of treatment, whereas error bars indicate the standard error of the mean. Differences between the four groups were analyzed using ANOVA with mixed-effect model. Differences in control versus FTY720 and control versus FTY720 plus trastuzumab were statistically significant. P value: * P < 0.05 and ** P < 0.01. ( b ) Tumor tissue sections were evaluated using TUNEL assays (green) and also counterstained with Hoechst 33,342 dye (blue, indicating nuclei). They were then examined using fluorescence microscopy. The top three tissue sections with the most abundant green signals in each group were presented. ( c ) Using the same tissue sections, cell membranes were outlined using HER2-antibody conjugating secondary antibody (red) and then examined using fluorescence microscopy. Bar, 100 μm. F plus T: FTY720 plus trastuzumab.

Article Snippet: After counterstaining with Hoechst 33,342 dye (blue, staining nuclei, Merck KGaA) and HER2 antibody/PE-Texas Red-conjugated secondary antibody (red, plasma membrane, Merck KGaA), the cells were examined using fluorescence microscopy .

Techniques: Control, TUNEL Assay, Fluorescence, Microscopy

Journal: Frontiers in Cell and Developmental Biology

Article Title: Patient-derived podocyte spheroids reveal new insights into the etiopathogenesis of Alport syndrome

doi: 10.3389/fcell.2023.1111424

Figure Lengend Snippet: Antibodies and reagents used in immunofluorescence (IF) and western blot (WB) analysis.

Article Snippet: Hoechst 33,258 , 16,756, Cayman , 2 μg/mL IF.

Techniques: Immunofluorescence, Western Blot

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Synthesis and evaluation of [ 18 F]FBNAF, a STAT3-targeting probe, for PET imaging of tumor microenvironment

doi: 10.1186/s41181-024-00276-w

Figure Lengend Snippet: Ex vivo autoradiography and immunohistochemical staining in a tumor section removed from a A549 tumor-bearing mouse administered [ 18 F]FBNAF. An autoradiogram was obtained from the tumor excised at 30 min after the injection of [ 18 F]FBNAF. Adjacent sections were used for immunohistochemistry with antibodies against STAT3 and pSTAT3. The results of Hoechst staining are also provided in the supporting information (Fig. S3). The scale bar represents 1000 μm

Article Snippet: Nuclear staining was performed by incubation with Hoechst 33,342 (Nacalai Tesque) for 10 min.

Techniques: Ex Vivo, Autoradiography, Immunohistochemical staining, Staining, Injection, Immunohistochemistry

Journal: BMC Complementary Medicine and Therapies

Article Title: Quercetin triggers cell apoptosis-associated ROS-mediated cell death and induces S and G2/M-phase cell cycle arrest in KON oral cancer cells

doi: 10.1186/s12906-025-04782-5

Figure Lengend Snippet: The KON cells treated for 24 ( a ) and 48 ( b ) h with quercetin at 25, 50, 100, 200, and 400 µg/mL showed changes. Using Hoechst 33,258 and AO double staining diacetate, the changes in the cytoplasm and nucleus were examined. KON cells were observed under a 20x fluorescent microscope. Red arrows denote apoptotic nuclei

Article Snippet: 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) (Sigma, USA), 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Thermo Fisher Scientific Inc, US), Fluorescein diacetate (FDA) (SolarBio ® , China), Hoechst 33,258 (SolarBio ® , China), Acridine Orange (AO) (SolarBio ® , China), Annexin V-FITC (BD Biosciences, San Diego, CA, USA), 2′,7′-Dichlorodihydrofluorescein diacetate (DCFDA) (Sigma, USA), Rhodamine 123 (Rho123) (SolarBio ® , China), Propidium iodide (PI) (SolarBio ® , China), the GF-1 Total RNA Extraction kit (Vivantis, Malaysia), ImProm-IITM Reverse Transcription System (Promega, USA), Taq polymerase (Vivantis, Malaysia), and VISafe green gel stain (Vivantis, Malaysia).

Techniques: Double Staining, Microscopy